Chromatography

Liquid chromatography tandem mass spectrometry (LC-MS) was carried out with a Shimadzu Prominence HPLC system and SCIEX 6500 Qtrap mass spectrometer. An aliquot of 10 µl of each sample was injected onto an Jupiter 4 µm C18 150 x 2.0 mm column at 40^oC for gradient separation. Mobile phases were: A, ddH₂O with 0.1% Formic Acid; 0.5% DMSO; and B, Acetonitrile with 0.1% Formic Acid; 0.5% DMSO. Gradient schedule started at 5% B and was linearly increased to 100% B at 2.5 minute, 100% B hold to 2.75 minutes, 5% B at 3.0 minutes and held at 5% B until 5.0 minutes to re-equilibrate. Time per injection was 5.0 minutes.

Sample Processing

1. Extraction Protocols:
   Extraction protocols for sample processing are carried out in the J. Edwin Blalock laboratory as part of the Lung Health Center. Please feel to contact Landon Wilson or Taylor Berryhill to make arrangements for extractions.

References

[1] Respir Res. 2009; 10(1): 38. Published online 2009 May 18. doi: 10.1186/1465-9921-10-38
N-α-PGP and PGP, potential biomarkers and therapeutic targets for COPD
Philip O'Reilly, corresponding author, Patricia L Jackson, Brett Noerager, Suzanne Parker, Mark Dransfield, Amit Gaggar and J Edwin Blalock

Resources

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